[Endovascular thrombectomy in patients with intracranial large artery occlusion and atrial fibrillation].

Objective: To investigate the safety and efficacy of mechanical thrombectomy in patients with atrial fibrillation complicated with acute intracranial arterial occlusion. Methods: Fifty-eight patients with atrial fibrillation complicated with acute intracranial arterial occlusion in the intervention group of East (Endovascular Therapy for Acute ischemic Stroke Trial) were analyzed. According to the TOAST (Trial of Org 10 172 in Acute Stroke Treatment) classification, patients were divided into ICAS (Intracranial Atherosclerotic Stenosis) group and cardiogenic embolism group. Clinical characteristics, treatment methods and clinical prognosis were compared between ICAS group and cardiogenic embolism group. Results: A total of 58 patients with atrial fibrillation complicated with acute intracranial arterial occlusion were included in this study, including 46 patients in the cardiogenic embolism group (79%) and 12 patients in the ICAS group (21%). The pre-hospital transport time in ICAS group was longer than that in cardiogenic embolism group (P<0.05).Patency rate in patients with atrial fibrillation complicated with acute intracranial arterial occlusion was 98.3% (57/58), The rate of patients with the 90-day function independent (mRS 0-2) was 51.7% (30/58). There were no statistically significant differences in functional independence, mortality rate, ICH and sICH at 90 days between the cardiogenic embolism group and the ICAS group. Conclusions: Mechanical thrombectomy is an effective method to treat patients with atrial fibrillation complicated with acute intracranial arterial occlusion.

Comparison of raw meat quality and protein-gel properties of turkey breast fillets processed by traditional or cold-batter mincing technology.

This study was conducted to evaluate the effects of cold batter mincing on meat quality and protein functionality, using turkey fillets that were chill-boned (CB) or hot-boned (HB) with crust-freeze-air-chilling (HB-»CFAC) at -12°C. For each of four replications, 48 toms (male) were raised and processed at Michigan State University Poultry Farm and Meat Laboratory, respectively. After evisceration, the turkeys were subjected to one of the four treatments: (1) traditional mincing of CB fillets after water immersion chilling (WIC); (2) cold batter mincing of WIC, CB, quarter-sectioned (»), and CB-»CFAC; (3) traditional mincing of HB-»CFAC fillets; and (4) cold batter mincing of HB-»CFAC fillets. Before mincing, the pH and R-values of turkey fillets in HB-»CFAC were higher and lower, respectively, than those in CB fillets. During cold-batter mixing, the initial batter temperatures at -1.5 to -2.1°C reached 1.5°C and 14°C at 6 and 12 min, respectively, and ended at 26 to 31°C at 24 min. During traditional mincing, the initial batter temperatures at 3 to 4°C increased by ∼10°C every 6 min, and ended at 32 to 35°C with higher batter temperatures seen for the 2% salt than the 1% salt batter. Dynamic rheological properties indicated that the cold-batter mincing showed elevated G' compared to the batters of traditional mincing, regardless of mixing time, indicating that the gel-setting temperature was reduced in the cold-batter mincing, potentially due to the different amounts of extracted protein and structural change. After cooking, improved cooking yield and protein functionality were observed in the batter of HB-»CFAC fillets than the batter of CB fillets as well as in the batter of 2% salt than the batter 1% salt (P < 0.05). These results indicated that HB-»CFAC fillets produced superior raw meat quality over the CB fillets, and cold batter mincing of HB-»CFAC fillets significantly improved protein functionality compared with the traditional mincing of CB fillets (P < 0.05).

The reduction of proprioceptors in the mesencephalic trigeminal tract nucleus after neonatal masseteric nerve transection; effect of brain-derived neurotrophic factor.

The effect of neonatal masseteric nerve transection on primary proprioceptors was examined in the mesencephalic trigeminal tract nucleus (Mes5) of the rat. At 72 h to 21 days after the injury, the number of Mes5 neurons decreased on the side ipsilateral to the transection. The means+/-SD of percentage proportion of ipsilateral/contralateral neurons at 72 h and 21 days were 69.9+/-7.5% and 58.2+/-14.6%, respectively. The application of brain-derived neurotrophic factor to the proximal stump of the masseteric nerve delayed the loss of Mes5 neurons at 72 h after the injury; the mean numbers+/-SD of ipsilateral and contralateral Mes5 neurons in injured animals with BDNF application was 553.6+/-61.9 and 558.4+/-55.3, respectively. Saline application had no effect on the injury-induced loss of Mes5 neurons; i.e., the mean numbers+/-SD of ipsilateral and contralateral Mes5 neurons were 367.3+/-72.5 and 543+/-33.5, respectively. These findings indicate that trigeminal primary proprioceptors are sensitive to the neonatal injury. The survival of proprioceptors during early postnatal period is probably dependent upon brain-derived neurotrophic factor in the trigeminal nervous system.

Investigation of the binding behaviors of isonucleoside-incorporated oligonucleotides with complementary sequences.

Oligonucleotides consisting of isonucleoside 2',5'-anhydro-3'-nucleobase-D-mannitol incorporated in 1'→4' linkage mode were synthesized. Their binding behaviors with complementary sequences were investigated via thermal denaturation and CD spectra. 6' O-methyl-2',5'-anhydro-3'-(thymin-1-yl)-D-mannitol incorporated oligonucleotide was also synthesized to investigate the effect of hydroxy groups of isonucleosides on duplex formation. The results showed that the 6'-OH free isonucleosidemodified oligonucleotide was able to form a B-like duplex with 3'→5' complementary native oligodeoxynucleotide in the 1'→4' direction. The free hydroxy group in the isonucleoside made a significant contribution to the affinity of the modified oligonucleotide to the complementary sequence, which was confirmed by molecular dynamics simulation.

Brain-derived neurotrophic factor-immunoreactive primary sensory neurons in the rat trigeminal ganglion and trigeminal sensory nuclei.

Immunohistochemistry for brain-derived neurotrophic factor (BDNF) was performed on the rat trigeminal ganglion (TG). The immunoreactivity (IR) was detected in 46% of TG neurons. These neurons were mostly small- or medium-sized (range, 149.7-1246.3 microm2; mean +/- SD = 373.4 +/- 151.6 microm2). A double immunofluorescence method also revealed that 54% of BDNF-immunoreactive (IR) neurons were immunoreactive for calcitonin-gene-related peptide. In addition, 93% of BDNF-IR TG neurons contained vanilloid receptor subtype 1. However, the co-expression of BDNF and vanilloid receptor 1-like receptor was very rare (less than 1%). In the trigeminal sensory nuclei, laminae II of the medullary dorsal horn was abundant in presumed BDNF-IR axon terminals. Such profiles were also detected in the dorsolateral part of the subnucleus oralis. The retrograde tracing and immunohistochemical methods demonstrated that BDNF-IR was common among cutaneous TG neurons (47%) but not tooth pulp TG neurons (13%). The present study indicates that BDNF-IR TG neurons have unmyelinated axons and project to the superficial medullary dorsal horn. It is likely that BDNF-containing neurons in both the trigeminal and spinal sensory systems have similarities in morphology and function. However, the content of BDNF in TG neurons probably depends on their peripheral targets. BDNF seems to convey nociceptive cutaneous input to the trigeminal sensory nuclei.

Calretinin-containing neurons which co-express parvalbumin and calbindin D-28k in the rat spinal and cranial sensory ganglia; triple immunofluorescence study.

The co-expression of calretinin with parvalbumin and calbindin D-28k was examined in the rat cranial and spinal sensory ganglia by triple immunofluorescence method. In the trigeminal and nodose ganglia, 9% and 5% of calretinin-immunoreactive neurons, respectively, also contained both parvalbumin- and calbindin D-28k immunoreactivity. These neurons had large cell bodies. In the trigeminal ganglion, they were restricted to the caudal portion. Such neurons were evenly distributed throughout the nodose ganglion. The co-expression could not be detected in the dorsal root, jugular or petrosal ganglia. Nerve fibers which co-expressed all the three calcium-binding proteins were observed in the inferior alveolar nerve but not the infraorbital nerve or palate. In the periodontal ligament, these nerve fibers formed Ruffini-like endings. These findings suggest that (1) the co-expression in trigeminal neurons is intimately related to their peripheral receptive fields; (2) the three calcium-binding proteins (calretinin, parvalbumin, calbindin D-28k) co-expressed in the trigeminal neurons may have mechanoreceptive function in the periodontal ligament.

Osteocalcin-immunoreactive neurons in the vagal and glossopharyngeal sensory ganglia of the rat.

Immunohistochemistry for osteocalcin (OC) was performed on the rat vagal and glossopharyngeal sensory ganglia. OC-immunoreactive (IR) neurons were detected in the jugular (10%), petrosal (11%) and nodose ganglia (6%). The cell size analysis demonstrated that OC-IR neurons were predominantly small to medium-sized in the jugular ganglion (mean+/-S.D.=356.3+/-192.2 microm(2), range=86.5-831.5 microm(2)). On the other hand, such neurons were medium-sized to large in the petrosal (mean+/-S.D.=725.6+/-280.7 microm(2), range=124.7-1540.4 microm(2)) and nodose ganglia (mean+/-S.D.=857.5+/-330.2 microm(2), range=367.1-1608.0 microm(2)). In the circumvallate papilla, OC-IR nerve fibers were located in the vicinity of taste buds. Some taste bud cells were also immunoreactive for the calcium-binding protein (CaBP). In the carotid body, however, OC-IR nerve fibers could not be detected. Retrograde tracing with fluorogold revealed that OC-IR nerve fibers in the circumvallate papilla mainly originated from the petrosal ganglion. These findings may suggest that OC-IR petrosal neurons have chemoreceptive function in the tongue.

Application of a bio-electrochemical reactor process to direct treatment of metal pickling wastewater containing heavy metals and high strength nitrate.

The fundamental performance of a bio-electrochemical reactor for the direct treatment of metal pickling wastewater was investigated experimentally. In the reactor, carbon anode and cathode were installed. On the cathode, denitrifying microorganisms were immobilized. Continuous experiments were carried out by feeding a synthetic wastewater containing nitrate and binary heavy metal ions, copper and lead, under different operating conditions. Acetate as well as the electric current was supplied at the minimum amount for stoichiometry of the dissimilatory denitrification reaction. The results indicated that the dissolved copper and lead removal, denitrification and neutralization could be achieved simultaneously in a single bio-electrochemical reactor. The dissolved heavy metals were removed by electrochemical deposition on cathode and by the other phenomena such as the formation of insoluble suspensions and the sorption on suspended bacterial sludge. Denitrification proceeded effectively with the utilization of both added acetate and hydrogen gas generated by electrolysis of water. The pH value increased up to around neutral due to the occurrence of denitrification in the reactor, although the influent pH was less than 3. The removal efficiencies of heavy metals and nitrate increased with increasing the current density. The applied electric current was indispensable for sustaining the stable treatment in the reactor.

VR1-, VRL-1- and P2X3 receptor-immunoreactive innervation of the rat temporomandibular joint.

Immunohistochemistry for vanilloid receptor subtype 1 (VR1), vanilloid receptor 1-like receptor (VRL-1) and P2X3 receptor was performed in the rat temporomandibular joint (TMJ). Blood vessels in the articular disk and capsule, the synovial membrane and the fibrous tissue around the condylar process were innervated by VR1- or P2X3 receptor-immunoreactive (ir) nerve fibers. However, VRL-1-immunoreactivity (ir) could not be detected in the TMJ. Retrograde tracing and immunohistochemical methods revealed that 25%, 41% and 52% of TMJ neurons in the trigeminal ganglion (TG) exhibited VR1-, VRL-1- and P2X3 receptor-ir, respectively. VR1-ir TMJ neurons were mostly small to medium-sized, whereas VRL-1- and P2X3 receptor-ir TMJ neurons were predominantly medium-sized to large. In addition, 73%, 28% and 44% of VR1-, VRL-1- and P2X3 receptor-ir TMJ neurons, respectively, coexpressed calcitonin gene-related peptide (CGRP)-ir. The present study suggests that the TMJ has abundant nociceptors which respond to vanilloid compounds, protons, heat and extracellular ATP.

[Experimental study on effect of jiawei yupingfeng san on microcirculation and plasma superoxide dismutase activity in old mice].

The effects of Jiawei Yupingfeng San on microcirculation and plasma SOD of old mice were observed. The results showed that the prescription could markedly improve old mice's blood flow of microcirculation, increase their speed of blood flow and raise their plasma TSOD and Cu, Zn-SOD activity, and there was very significant difference compared with control (P < 0.001). The results suggested that this prescription had the effects of improving the quality of life and delaying the aging of organism.